Normal and Injured Spinal Cord

Axonal tracts in the spinal cord are covered by myelin, an insulating lipid-based substance, which is of paramount importance for the correct signal transmission through the axons and is involved is a series of pathological and traumatic states. 

CARS microscopy was used for the study of axonal myelin under physiological conditions in spinal cord, using white matter strips isolated from the spinal cord of guinea pigs and kept alive in oxygen-bubbled Krebs solution. Both forward-and epi-detected CARS were used to probe the axon bundles and resolve detailed structures such as nodes of Ranvier. In addition, simultaneous CARS microscopy of myelin and TEPF imaging of intra- and extra-axonal calcium was demonstrated [58]. 

and Terhune, R.W. (1965) Study of optical effects due to an induced polarization third order in electric field strengfh. Phys. Rev., 137, A801-A818. 

(1974) Coherent anti-Stokes raman-spectroscopy. Appl. Phys. Lett., 25, 

Muller, M. and Zumbusch, A. (2007) Coherent anti-Stokes Raman scattering microscopy. ChemPhysChem, 8, 2156-2170. 

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