Nonspecific binding correct antigen

Fig. 5.1 Sources of nonspecific binding. Incubation with antibodies should have only antibody binding to the correct antigen. Nonspecific binding can result from charged groups that bind proteins including antibodies. The tissue can have Fc receptors that bind to the Fc region of any antibody. Some tissues can have exposed antibodies...

Fig. 5.2 Examples of nonspecific antibody binding. During immunocytochemistry incubations nonspecific binding occurs. The correct antigen will bind the primary antibody correctly. Charged groups will bind to any protein or antibody such as the 1° antibody. The Fc receptor will bind the first antibody that comes in contact here with the 1° antibody. Any endogenous antibodies are likely to bind only to secondary antibodies made against the species of the animal...

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