Nonseparation or homogeneous assays

The ability to run assays that require a separation step becomes limited as assay volumes are reduced in higher density array plates. Therefore, mix-and-read or homogeneous screening formats are essential to miniaturization, requiring only a series of additions to perform the screen. Examples of homogeneous assay formats that have been developed for low volume screens include prompt fluorescence, FRET including time-resolved FRET, fluorescence polarization, and laser scanning fluorimetry. 

IDENTIFICATION OF RECEPTOR ANTAGONISTS FOR CHEMOKINE RECEPTOR AND BRADYKININ-I BY SCREENING A 150,000-MEMBER COMBINATORIAL LIBRARY 

FIGURE 6 Frequency of each substituent at RI, R2, R3, and R4 in structures active against CXCR2 (upper panel) and BK-I (lower panel). Active structures predicted from the encoded library, against either CXCR2 or BK-I receptors, are evaluated in terms of substituents at each point of variation. The frequency with which each substituent is found in the active structures is indicated in comparison to all other possibilities at that position RI = 31 R2 = 31 R3 = 4 R4 = 38. 

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