Nonaqueous environments biocatalysts

This chapter reviewed the methodology required to insert proteins and operate biocatalytic reactions in nonaqueous environments. Nonaqueous enzymology has opened the door to new and exciting avenues in the chemical sciences, such as enantiomerically pure products. Ongoing research will continue to produce more effective biocatalysts and operation procedures for biocatalytic reactions. In conclusion, nonaqueous biotechnology will continue to grow in the upcoming years. 

Lipases are an important class of industrial enzymes and are used as chiral catalysts in a variety of reaction types. In organic synthesis, lipases are one of the most widely utilized and versatile biocatalysts. Lipases maintain activity in a nonaqueous environment, a property resulting in several advantages over other classes of enzymes. The water-insoluble enzymes and products can be readily recovered and the enzymes recycled. The absence of water can decrease the likelihood of side reactions and decrease product or substrate inhibition [6]. 

---