Non-stirred microcarriers

A layer of microcarriers can be arranged on the growth surface of a flask or roller bottle to increase the surface area. In this case the 

1) to a newly seeded flask add sufficient microcarriers to almost cover the bottom of the flask, or 

2) to a flask in which the cells are 50% confluent add microcarriers to cover about half the area of the flask. If the culture vessel is a roller bottle, rotation should be very slow (0.1 r.p.m.) until the cells and beads are attached. 

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