Non-isotopic tracer

The MIP-ILAs based on a direct format will be described elsewhere. ILAs based on indirect methods will be reviewed in this chapter. Like immunoassays, the first MIP-ILA, reported in 1993 by the Mosbach group [25] for the analysis of theophylline and diazepan, was based on a radioactive tracer. Since this work, many other indirect MIP-ILAs have been developed using non-isotopic tracers such as fluorophores, enzymes, or electroactive probes. 

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