NOESY presat

Fig. 4.9 (A)-(C) H spectra recorded at 500 MHz on a 4 mM solution of a compound in a 60 40 (v/v) mix of D2O and CH3CN. (A) Simple pulse and collect ID H spectrum showing the C satellites of acetonitrile (B) NOESY presat spectrum with presaturation of the MeCN signal apphed during the relaxation delay (2 s) and during the mixing time (200 ms) using a field of 90 Hz (C) Excitation sculpting sequence using a 1 s presaturation of water and a selective proton JC pulse of 4.25 ms. Compare the spectral region around the residual acetonitrile resonance in (B) and (C) - excitation sculpting results in the obliteration of far less of the spectrum near the suppressed solvent resonance, which in this case contains many solute resonances.

Figure 6 Presat, WEFT/NOESY, and FLIPSY. The typical (A) presat, (B) WEFT/NOESY and (C) FLIPSY. Any 90 and 180 hard pulses are identified, respectively, by narrow and wide hollow rectangles, respectively, with relative phases indicated inside the shape (where appropriate). Outlined Gaussian shapes indicate selective pulses while gradients are represented on their own horizontal line by filled shapes. For full phase cycle discussions please refer to the referenced manuscripts. The 45 phase shifted pulse discussed in the text is indicated by the asterisk in the NOESY sequence and an V in the FLIPSY shows the variable flip angle pulse. The modified FLIPSY contains a homo-spoil gradient just after the presaturation period.

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