Nitrosomonas europea

Juliette LY, MR Hayman, DJ Arp (1993) Inhibition of ammonia oxidation in Nitrosomonas europeae by sulfur compounds thioethers are oxidized to sulfoxides by ammonia monooxygenase. Appl Environ Microbiol 59 3718-3727. 

The first test case was the ferrous high-spin state (Fe, S = 2) in the picket-fence porphyrin acetate complex [Fe(CH3COO)(TPpivP)] [13, 23], which is a model for the prosthetic group termed P460 of the multiheme enzyme hydroxyl-amine oxidoreductase from the bacterium Nitrosomonas europeae. Both the picket-fence porphyrin and the protein P460 exhibit an extraordinarily large quadrupole splitting, as observed by conventional Mossbauer studies [56]. 

Vanelli, T., Logan, M., Arciero, D. M. Hooper, A. B. (1990). Degradationofhalogenated aliphatic compounds by the ammonia-oxidizing bacterium Nitrosomonas europeae. Applied and Environmental Microbiology, 56, 1169-71. 

Figure 7 Schematic representation of the of di-heme peroxidases from Pseudomonas aeruginosa (PAP) and Nitrosomonas europea (NEP) showing key residues involved in catalysis. (This figure was generated from coordinates of 1EB7 and IIQC deposited in the Protein Data Bank)...

The role of oxygen concentration has already been noted in a variety of contexts (1) in the regulation of the synthesis of peroxidases in white-rot fungi (Section 4.4.4), (2) in determining the outcome of the degradation of the trichloromethyl group in a substituted pyridine by Nitrosomonas europeae... 

Chemoautotrophs are microbes which obtain energy by catalyzing an exergonic chemical reaction (commonly at interfaces between aerobic and anaerobic environments) and which produce biomass by fixing inorganic carbon. Nitrosomonas europea oxidizes ammonia and uses rubisco and the Calvin Cycle to fix carbon (the most common sulfide-oxidizing organisms also use the Calvin Cycle). Its isotopic characteristics could therefore, be expected to be similar to those of other aerobic, prokaryotic autotrophs such as Synechocystis. As shown in Table 5, this expectation is fulfilled. 

Hunik JH, van den Hoogen MP, de Boer W, Smit M, Tramper J (1993) Quantitative determination of the spatial distribution of Nitrosomonas europea and Nitrobacter agilis cells immobilized in kappa carrageenan gel beads by a specific fluorescent antibody labeling technique. Appl Environ Microbiol 59(6) 1951-1954... 

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