Nitrogenase structure

Peters, J.W. and Szilagyi, R.K. (2006) Exploring new frontiers of nitrogenase structure and function, Curr. Opin. Chem. Biol., 10, 101-108. 

Figure 24-4 Sequence of nif genes of Klebsiella pneumoniae.56 These precede the his operon directly at the right side. The nitrogenase structural genes are marked with green.

Ohrme-Johnson, W.H. (1992) Nitrogenase structure where to now Science 257, 1639-1640. 

B. K. Burgess, Nitrogenase structure, function and genetics, in Molybdenum Enzymes, Cofactors and Model Systems , eds. 

J.W. Peters, K. Fisher, and D.R. Dean. 1995. Nitrogenase structure and function A biochemical-genetic perspective... 

Mutant strains of A. chroococcum carrying deletions of both Mo and V nitrogenase structural genes are unable to grow on N2 ill). In contrast, comparable strains of A. vinelandii can grow provided neither Mo nor V is added to the growth medium (12). This ability is due to the presence of the third nitrogenase in A. vinelandii. 

Orme-Johnson, W. Nitrogenase Structure Where to Now Science 257, 1639-1640 (1992). [Thoughts about nitrogen fixation based on the determination of the structure of nitrogenase by X-ray crystallography]. 

Other metals associated with the MoFe proteins in significant amounts are Mg, Cu, Ca, and Zn with Kpl, Ca, and Mg with Cpl and Zn with Avl. Presumably these are adventitious associations, with the possible exception of Mg ", which may be involved in the protection of Azotobacter nitrogenase complex against damage by Oj (Scherings, 1978). No role has been attributed to the other metals, neither in nitrogenase structure nor mechanism. 

Mo-Nitrogenase Structure of the 2 1 Fe Protein MoFe Protein Complex. Three approaches have been used to form a stable complex of the Fe protein with the MoFe protein. First, because the Fe protein is a member of the nucleotide switch protein family, the AIF4 plus MgADP trap was applied to Mo-nitrogenase (28 PDB code 1N2C). The result is a stable nitrogenase complex consisting of two Fe proteins bound to one MoFe protein. A second, very... 

Orme-Johnson, W. H. (1992) Nitrogenase Structure Where To Now Science 257,1639-1640. 

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