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Nitrogen bases reversed-phase HPLC

Figure 1.5. A reversed-phase HPLC chromatogram of three organic components eluting in the order of polar first and nonpolar last. The basic pyridine peak is tailing due to a secondary interaction of the nitrogen lone-pair with residual silanol groups of the silica based bonded phase. Figure reprinted with permission from reference 8, Chapter 2. Figure 1.5. A reversed-phase HPLC chromatogram of three organic components eluting in the order of polar first and nonpolar last. The basic pyridine peak is tailing due to a secondary interaction of the nitrogen lone-pair with residual silanol groups of the silica based bonded phase. Figure reprinted with permission from reference 8, Chapter 2.
The second example is a hplc experiment in the reversed-phase mode involving the separation of nitrogen bases. Here the stationary phase selectivity is altered for some bases by the addition of a nickel complex to the mobile phase. As demonstrated by LochmUller and Hangac (6) these uncharged, coordinatively unsaturated complexes interact selectively with some bases but not others. Enhanced resolution is acheived at low concentrations (.0001 M) because some bases undergo a 10-fold Increase in retention. The result is... [Pg.43]

See other pages where Nitrogen bases reversed-phase HPLC is mentioned: [Pg.399]    [Pg.118]    [Pg.43]    [Pg.257]    [Pg.246]    [Pg.263]    [Pg.435]    [Pg.374]    [Pg.246]    [Pg.311]    [Pg.5]    [Pg.231]    [Pg.1891]    [Pg.1252]    [Pg.139]   
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Nitrogeneous bases

Nitrogenous bases

Reverse-phase HPLC

Reversed-phase HPLC

Reversible bases

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