Niflumic acid, synthesis

Synthesis Condensation of 2-chloronicotinic acid with 3-trifluoromethylaniline or reaction of 2-aminonicotinic acid with 1-bromo-3-trifluoromethylbenzene yields niflumic acid (Sherlock and Sperber (Schering Corp.), 1967 Faure and Hoffman (Labs. U.P.S.A.), 1968 Kleemann et al., 1999). 

Good use is made of this chemistry in the synthesis of nifluminic acid 69, an analgesic with a pyridine ring. Disconnection of the C—N bond suggests a 2-chloropyridine 70 starting material easily derived from nicotinic acid 60 and a simple aromatic amine 72 available by functionalisation of trifluorobenzene 74. 69 Is actually available as niflumic acid from Aldrich. 

Effects of Inhibitors of Eicosanoid Synthesis Enzymes on TNF-a Production. To further investigate how different eicosanoids affect TNF-a production, THP-1 cells were preincubated with COX inhibitors, and then stimulated with EPS. Indomethacin (5 [iM), which is a nonselective COX inhibitor, did not affect EPS-stimulated TNF-a production of THP-1 cells (Fig. 6). In addition, niflumic acid (2 iM), which is a selective inhibitor of COX-2, and aspirin (2 mM),... 

---