Nicotinamide adenine dinucleotide spectrophotometric determination

Ahmad, I., and HeUebust, J. A. (1989). A spectrophotometric procedure for measuing oxoglutarate and determining aminotransferase activities using nicotinamide adenine dinucleotide phosphate-hnked glutamate dehydrogenase from algae. Anal. Biochem. 180, 99—104. 

Lactose can also be measured enzymatically by hydrolysis in the presence of nicotinamide adenine dinucleotide (NAD) which is reduced to NADH (see eqns [1] and [2]). The amount formed is determined spectrophotometrically at 340 nm ... 

Later, an enzymatic method based on oxidation of ethanol by alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD) followed by spectrophotometric analysis was reported. Current methods for detecting ethanol in body fluids are predominantly based on physicochemical techniques. A gas-liquid chromatographic (GLC) method is the most widespread because it is easy, rapid, and has high specificity and accuracy. Analytical methods used to determine alcohol in body fluids are siunma-rized in Table 1. 

Enzymatic reactions UV-visible absorption spectrophotometric determination of enzyme inhibitors is much more popular than that of enzyme activators. Drugs such as neostigmyne, chloropromazine, and cisplatin can be determined by inhibition of soluble or bound enzymes, viz., acetylcholine sterase. Nicotinamide adenine dinucleotide hydrogenase (NADH), and peroxidase, respectively. 

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