Nicotinamide adenine dinucleotide kinase

Staining Applications Cells oxidized nicotinamide adenine dinucleotide kinase sperms "... 

Mailloux, R. J. Singh, R. Appanna, V. D. In-gel activity staining of oxidized nicotinamide adenine dinucleotide kinase by blue native polyacrylamide gel electrophoresis. Anal. Biochem. 2006, 359, 210-215. 

Abbreviations FASN, fatty acid synthase ACC, acetyl-CoA-carboxylase ACL, ATP-citrate lyase NADPH, nicotinamide adenine dinucleotide phosphate MAT, malonyl acetyl transferases KS, ketoacyl synthase KR, p-ketoacyl reductase DH, p-hydroxyacyl dehydratase ER, enoyl reductase TE, thioesterase ACP, acyl carrier protein VLCFA, very long chain fatty acids ELOVL, elongation of very long chain fatty acids SCDl, stearoyl-CoA desaturase-1 AMPK, AMP-activated kinase ME, malic enzyme FASKOL, liver-specific deletion of FAS PPARa, Peroxisome Proliferator-Activating Receptor alpha HMG-CoA, 3-hydroxy-3-methyl-glutaryl-CoA SREBP, sterol response element binding protein SIP, site-one protease S2P, site-two... 

Kinase (phosphorylating), nicotinamide adenine dinucleotide 429b, 2095b, 4249, 4977... 

Scheme 1.—The Reactions Involved in the Enzymic Determination of (a) Glycerol, (b) Erythritol, (c) Glycolaldehyde, and (d) and (e) Formic Acid. [Abbreviations ATP, adenosine 5 -triphosphate ADP, adenosine S -pyrophosphate NAD and NADH, the oxidized and reduced forms of nicotinamide adenine dinucleotide PEP, enolpyruvate phosphate Pi, inorganic orthophosphate GK, glycerol kinase GDH, u-glycerophosphate dehydrogenase EK, erythritol kinase PK, pyruvate kinase YAD, yeast alcohol dehydrogenase FS, formyltetrahydrofolate synthetase FD, formate dehydrogenase and NR, nitrate reductase.]...

Brevibacterium ammoniagenes cells immobilized by copolymerization with acrylamide have been used in column form for the continuous production in high yield of pure nicotinamide adenine dinucleotide phosphate, for the continuous production of L-malic acid from fumaric acid, and for study of the NAD-kinase activity of the immobilized cells. The continuous production of L-malic acid has also been achieved with Brevibacterium flavum cells immobilized by gelation in k-carrageenan. ... 

Li et al. over expressed Nicotinamide Adenine Dinucleotide (NAD) kinase to enhance the accumulation of PEIB in recombinant E. coli harboring PEIB synthesis pathway by an accelerated supply of NADPH, one of the important factors affecting PHB production. The study revealed that NAD kinase in E. coli harboring the PHB synthesis operon could increase the accumulation of PHB to 16-35% weight compared... 

Enzyme purification was the first application of bioaffinity chromatography [23] and remains an important use of this technique. In this type of separation, ligands, such as enzyme inhibitors, coenzymes, or cofactors, are used to purify and separate enzymes [25]. For instance, in 1968 and the first report of "modem" affinity chromatography, Cuatrecasas, Wilchek, and Anfinsen employed specific enzyme inhibitors to selectively isolate enzymes [1,4]. A more recent example was the use of a support containing flavin mononucleotides for the purification of flavin adenine dinucleotide synthetase [26]. Other examples have included the use of mono-, di-, and triphosphate nucleotides for the purification of kinases and the use of nicotinamide adenine dinucleotide for the isolation of dehydrogenases [26,27]. 

Figure 2 NAD metabolism. Tip = tryptophan, 3-HK = 3-hydroxykynurenine, 3-HA = 3-hydroxyanthranilic acid, ACMS = a-amino-P-carboxymuconate- -semialdehyde, AMS = a-aminomuconate- -semialdehyde, NaMN = nicotinic acid mononucleotide, NMN = nicotinamide mononucleotide, NaAD = nicotinic acid adenine dinucleotide. For other abbreviations, see Figure 1. (1) tryptophan oxygenase [EC 1.13.11.11], (2) formy-dase [EC 3.5.1.9], (3) kynurenine 3-hydroxylase [EC 1.14.13.9], (4) kynureninase [EC 3.7.1.3], (5) 3-hydroxyanthranilic acid oxygenase [EC 1.13.11.6], (6) nonenzymatic, (7) aminocarboxymuconate-semialdehyde decarboxylase [EC 4.1.1.45], (8) quinolinate phos-phoribosyltransferase [EC 2.4.2.19], (9) NaMN adenylyltransferase [EC 2.7.2.18], (10) NAD synthetase [EC 6.3.5.1], (11) NAD kinase [EC 2.7.1.23], (12) NAD" glycohydro-lase [EC 3.2.2.5], (13) nicotinamide methyltransferase [EC 2.2.1.1], (14) 2-Py-forming MNA oxidase [EC 1.2.3.1], (15) 4-Py-forming MNA oxidase [EC number not given], (16) nicotinamide phosphoribosyltransferase [EC 2.4.2.12], (17) NMN adenylytransferase [EC 2.7.71], (18) nicotinate phosphoribosyltransferase [EC 2.4.2.11], (19) nicotinate methyltransferase [EC 2.7.1.7], and nicotinamidase [EC 3.5.1.19]. Solid line, biosynthesis dotted line, catabolism.

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