Newer Procedures for Separation of Lipids

Every one of these principles of separation can be applied also in thin-layer chromatography, which usually gives better separations in shorter time the method has thus rapidly become iudispensable in lipid chemistry. The efficiency and scope of TLC may be extended still further by combination with other separation techniques. Adsorption TLC and partition chromatographic procedures, i.e., reversed phase partition TLC, paper or gas chromatography, should be used consecutively. Mixtures of compounds which cannot be fractionated by adsorption chromatography can usually be separated by reversed phase partition chromatography and vice versa. 

The following types of lipid fractionation have been accomplished by TLC  

Separation according to compound class, fractionation of vinylogous series, separation according to degree of unsaturation, separation of cis4rans isomers and of positional isomers. 

---