New advances in the theory of electrophoretic techniques

The objectives of each theoretical approach are not only the explanation of the experimental results or failures of practice but also the prediction of new possibilities to increase the sensitivity, separation capacity and velocity of the chromatographic procedure under investigation. Numerous theoretical reviews deal with the problems of the CE separation technique. In recent years the methods to enhance the precision in CE by the modification of operational parameters [113], the theory and methodological improvements of sample stacking of cationic and anionic solutes in CE [114-116], and the results and difficulties of the application of conductivity detection in CE technologies [117] have been reviewed. 

The peak distortion in CZE electrophoregrams by electromigration dispersion has been modelled and the peak centre was related by 

To facilitate calculations, eqns 1.31-1.33 were converted from time to distance dimensions. It was established that the total variance of the peak includes the variance due to diffusion and due to electromigration diffusion  

The results of theoretical calculations were compared with the concrete measurements using paraquat and 4-aminopyridine as model compounds. In the case of paraquat the fitness of the calculated values to the theoretical ones (RES) was expressed by 

It has been found that the equations describe correctly the peak shape determined experimentally, and can be applied for the prediction of peak distortion [118]. 

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