Neuron axon, micrograph

Fig. 14.11 Case No. 5 correct experiment. The spinal cord white matter has only neuronal axons and no neuronal cell bodies. The following micrographs are pairs from double-label experiments showing the white matter, (a) The cells labeled with rabbit anti-neurofilament and goat anti-rabbit 488 fluorophore showed axons, (b) The same filed as a showed no labeling for HuC as expected, (c) Incubation with no rabbit anti-neurofilament and with mouse anti-HuC followed by both 2° antibodies showed no label for neurofilament as expected, (d) Same field as (c) with no label in the HuC channel for axons, (e) Incubation with rabbit anti-neurofilament and without mouse anti-HuC and both 2° antibodies showed the labeling for axons, (f) Same field as (e) with no labeling in the HuC channel...

Figure 19.6 Micrographs of hippocampal neurons on FC membrane after (a) 4 hours, (b) 4 days (c) 16 days of culture. The arrows in a) indicate the emerging processes from the cell circumference the arrows in b) indicate the (black) axon and the dendrites (white).

Fig. 8. Effects of disruption of endophilin and amphiphysin interactions on clathrin-mediated endocytosis at the reticulospinal synapse. (A) Electron micrograph of the lateral side of the active zone in a control synapse stimulated at 5 Hz. Note the presence of clathrin-coated pits with different shapes. (B) Electron micrograph of the comparable area of a synapse in an axon that was stimulated at 5 Hz for 30 min after injection of endophilin antibodies. Note the pocket-like membrane expansions (arrows) at the margin of the synaptic area and the appearance of numerous shallow coated pits (arrows). (C) A synapse in an axon which was stimulated at 0.2 Hz for 30 min after injection of a fusion protein containing the SH3 domain of amphiphysin linked to GST. Note the accumulation of constricted coated pits around the active zone. Scale bar, 0.2 pm. B, modified from Ringstad et al. (1999), Neuron 24, 143-154, with permission copyright is held by Cell Press. C, modified from Shupliakov et al. (1997a) Science 276 259-263, with permission copyright 1997 AAAS.

Fig. 8. CCK in the MOB. A-C. Silver-intensified CCK-immunohistoehemical staining in the main olfactory bulb. CCK-immunoreactive neurons are located mainly in the superficial one-third of the EPL the majority of these CCK-positive neurons are tufted cells. The apical and secondary dendrites of these cells are well delineated in the higher-power micrographs of (B) and (C). Thin axon-like processes course toward the ll L. In addition to the staining of cell bodies and dendrites, there is a dense, uniform CCK-like immunoreactive band consisting of terminal-lie puncta restricted to the IPL. Calibration bar in A = 500 fira, bar in B = 100 /tm, and bar in C = 60 /mi.

A EXPERIMENTAL FIGURE 17-35 Fibrous proteins help localize synaptic vesicles to the active zone of axon terminals. In this micrograph of an axon terminal obtained by the rapid-freezing deep-etch technique, synapsin fibers can be seen to interconnect the vesicles and to connect some to the active zone of the plasma membrane. Docked vesicles are ready to be exocytosed. Those toward the center of the terminal are in the process of being filled with neurotransmitter. [From D. M. D. Landis et al., 1988, Neuron 1 201.]... 

Figure 3. Interference contrast micrograph of a 1-fim-thick section of the lamina that shows photoreceptor axon terminals surrounding the paired mono-polar neurons in each cartridge.

Figure 7. Fluorescence micrograph of a 1-pm-thick section of the lamina of a male fly that shows stained photoreceptor axon terminals cut parallel to their long axis. Gap junctions between the six peripheral cells occur in the outer 10 pirn of the axon terminal, just below the somas of the monopolar neurons. Because recorded cells are located in reference to the corneal facet matrix, the location and orientation of that cell in the underlying neuropil is predetermined, so it can be sectioned in any given plane and recovered for histological and histochemical examination.

Micrograph of one of the 21 midbody ganglia of a leech. All cell bodies of the 400 neurons, including two large serotonergic Retzius cells, are visible. Cell bodies and axons are stained with an antibody to the intermediate filament protein filarin. Courtesy of Kristen M. and Jorgen Johansen. (See p. 1762.)... 

Fig. 2, Low (A) and high (B) m ification views of a wholemount of a Chorex destructor ( Yabbie ) embryo (65% development), immunolabeled for GABA, using DAB as a chromogen. Arrows indicate labeled neuronal somata arrowheads indicate labeled axons. Many labeled elements are out of the plane of focus of the microscope. Scale bars, 100 pm. Micrographs kindly provided by Renate Sandeman, University of New South Wales.

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