Net C3-Carboxylating Flux in vivo

To determine the in vivo fluxes. Metabolic Flux Analysis techniques combining metabolite balancing and stable isotope labelling must be applied. Metabolite balancing provides the first step in flux quantitation by using mass balances of intracellular metabolites at steady state [28,29]. Thus, for the anaplerotic node 

Bakg + Bqaa- Abbreviations T, activity of the citric acid dele P, activity of the oxidative pentose phosphate pathway Glc6P, glucose 6-phosphate Fru6P, fructose-6-phosphate Gra3P, glyceraldehyde 3-phosphate PEP, phosphoenolpyruvate PYR, pyruvate AcCoA, acetyl coenzymeA OAA, oxaloacetate, AKG, 2-oxoglutarate 

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