NECEEM-Based Selection of Aptamers

Time window for coiiection of smart aptamers with predefined /foff  

Browser et al. snggested a CE-based procednre for aptamer selection, termed CE-SELEX, which is similar to NECEEM-based SELEX bnt does not use the kinetic capabilities of NECEEM (Mendonsa and Bowser, 2004, 2005 Mosing et al., 2005). 

Second, the migration time of the target is determined using UV detection. A sample of commercial PFTase shows four distinct peaks with migration times 

Essential steps of NECEEM-based aptamer development. See the text for 

Non-SELEX Aptamers for h-Ras protein were selected through 

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