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Nanoscale DNA analysis

Enzyme microreactors have also received wide application not only in the analysis but also in the synthesis of DNA. A nanoscale platform for real-time single-molecule sequencing by recording the DNA synthesis process by an immobilized DNA polymerase has been developed and has been covered by US patent application number 7,329,492 [62]. The process relies on fluorescence resonance energy transfer (FRET) between the fluorescence receptor moiety on the dNTPs and the fluorescent dye attached to the donor DNA that emits an enhanced signal upon nucleotide incorporation. Immobilization of polymerase enables increased sensitivity detection as the DNA strand extends imattached. [Pg.308]

See other pages where Nanoscale DNA analysis is mentioned: [Pg.1527]    [Pg.1529]    [Pg.1531]    [Pg.1533]    [Pg.1535]    [Pg.1537]    [Pg.1539]    [Pg.1541]    [Pg.1527]    [Pg.1529]    [Pg.1531]    [Pg.1533]    [Pg.1535]    [Pg.1537]    [Pg.1539]    [Pg.1541]    [Pg.428]    [Pg.115]    [Pg.128]    [Pg.231]    [Pg.540]    [Pg.629]    [Pg.894]    [Pg.215]    [Pg.306]    [Pg.446]    [Pg.495]    [Pg.10]    [Pg.155]    [Pg.68]    [Pg.473]    [Pg.552]    [Pg.207]    [Pg.1325]    [Pg.378]    [Pg.104]    [Pg.184]    [Pg.822]    [Pg.537]    [Pg.438]    [Pg.73]    [Pg.184]    [Pg.450]    [Pg.153]    [Pg.131]   


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Nanoscales

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