Nanoparticles click chemistry coupling

Figure 17.15 The small carboxylate-alkyne compound 4-pentynoic acid can be used to modify proteins at their amine groups with EDC to provide alkyne sites for click chemistry-mediated conjugation. The subsequent reaction of an azido-PEG-modified gold nanoparticle with the alkynyl-protein in the presence of Cu1+ yields the triazole-coupled protein.

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