NADP, complex with glutathione

The reactions in Eq. (10), proceeding to the right from E, are part of normal catalysis as shown in Fig. 10 (SI, S3). The association of E with NADP leads to a dead end complex. In the reaction of yeast glutathione reductase with NADPH, EH,-NADPH appears to be formed in the dead time of the rapid reaction spectrophotometer (co. 3 msec) when observation is at 540 nm (344), however, if 3.4 /iM EH,(free) is mixed with 20 fiM NADPH, Eq. (11), a minimum rate of complex formation of... 

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