Myosin light chains steady state

Figure 11.19 A schematic illustration of the application of fluorescence anisotropy measurements to study orientation changes in skeletal muscle fibres. A rhodamine-based fluorescent probe is bound to the regulatory light chain (RLC) of the myosin head. Changes in probe orientation directly related to changes in the steady-state orientation of the emission transition dipoles (yellow arrows) of the fluorescent probes in the laboratory frame which are monitored by fluorescence anisotropy. Stretches of the muscle fibre cause the transition dipoles to tilt towards the fibre axis with a corresponding decrease in the fluorescence anisotropy...

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