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Myosin light chains calcium free

Mamar-Bachi, A. Cox, J.A. Quantitative analysis of the free energy coupling in the system calmodulin, calcium, smooth muscle myosin light chain kinase. Cdl Calcium 1987, 8, 473-482. [Pg.371]

Himpens B, Matthijs G, Somlyo AV, Butler TM, Somlyo AP (1988) Cytoplasmic free calcium, myosin light chain phosphorylation, and force in phasic and tonic smooth muscle. J Gen Physiol 92 713-729... [Pg.126]

Fig. 6. Myosin light chain kinase (MLCK) is phosphorylated during microcystin-evoked contraction in both control and PDBu downregulated tissues. Control and downregu-lated tissues were incubated in calcium-free solution containing 10 mM EGTA and then stimulated with 1 pM microcystin for 1 hr in the presence of P-ATP, homogenized, and subjected to immunoprecipitation with an antibody against MLCK. A Autoradiograph of the immunoprecipitate of control (C) and downregulated (D) tissue. B Western blot of the same membrane for MLCK. Representative of three experiments. Autophos-phorylated MLCK is active in solution even in the absence of calcium (Tokui et al. 1995 Andrea and Walsh, personal communication 1997) From Walker et al. 1998. Fig. 6. Myosin light chain kinase (MLCK) is phosphorylated during microcystin-evoked contraction in both control and PDBu downregulated tissues. Control and downregu-lated tissues were incubated in calcium-free solution containing 10 mM EGTA and then stimulated with 1 pM microcystin for 1 hr in the presence of P-ATP, homogenized, and subjected to immunoprecipitation with an antibody against MLCK. A Autoradiograph of the immunoprecipitate of control (C) and downregulated (D) tissue. B Western blot of the same membrane for MLCK. Representative of three experiments. Autophos-phorylated MLCK is active in solution even in the absence of calcium (Tokui et al. 1995 Andrea and Walsh, personal communication 1997) From Walker et al. 1998.
See other pages where Myosin light chains calcium free is mentioned: [Pg.50]    [Pg.75]    [Pg.82]    [Pg.558]    [Pg.238]    [Pg.557]    [Pg.1141]    [Pg.2366]    [Pg.559]    [Pg.558]   
See also in sourсe #XX -- [ Pg.326 ]



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