Myelin bound cerebrosides

We obtained unexpected findings using this method to study myelin the oxidation by galactose oxidase of myelin-bound cerebrosides could not be detected. The oxidation did not occur either with the intact spinal cord preparation, with isolated myelin, or even with lyophilized myelin. In one experiment, lyophilized myelin containing 5 mg each of dry weight was incubated with 100, 200, and 500 units of galactose oxidase for 60 min at room temperature, and no cerebroside oxidation occurred. 

To further examine the inability of galactose oxidase in oxidizing myelin-bound cerebrosides, one mg each of lyophilized... 

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