Multienzyme systems same substrate

In one type of study, the HPLC assay method has been applied to the question of assaying the activity of two enzymes that can use the same substrate but form different products. In another, the HPLC method was applied to the case of related two different enzymes that can use the same substrate and form the same product. It was also applied to the case a single enzyme that can use either of two substrates to form two different products. In a separate application, the so-called reconstitution approach, a multienzyme pathway is reconstructed by the addition of enzymes one after the other to form a multienzyme complex and to thereby reconstitute a naturally occurring multienzyme complex. And finally, this approach allows us to use the HPLC method to study an intact, naturally occurring multienzyme system. 

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