Multidimensional signal, chemical analysis

A comparably simple approach uses perturbations of the target s chemical shifts to confirm binding. Multidimensional spectra are usually used, as signal overlap obscures the analysis in ID spectra. In these spectra, the displacement of peaks due to the binding event is monitored. Obviously, the use of 2D- or 3D spectra results in an increased experimental time. As an additional feature of this technique, the dissociation constant may be extracted from the experiment. This analysis is possible if the displacement is related to the concentration of the ligand via a titration. Of course, these experiments require a reference spectrum without the ligand. 

---