Multi combinatorial biocatalysis

In vitro multi-enzyme systems are set up by the combination of enzyme modules including pathway and even pathway-unrelated enzymes. Also, the synthesis of saccharides in combination with de novo enzymatic sugar synthesis can be accomplished. This so-called combinatorial biocatalysis can be performed in sequential reactors or in a one-pot reaction vessel which challenges further reaction engineering for optimization. Even the combination of an enzyme module with a chemical... 

Table 5.1 Multi-enzyme systems for the synthesis of glycoconjugates by the combination of enzyme modules in combinatorial biocatalysis and combinatorial biosynthesis.

I 5 Multi-Enzyme Systems for the Synthesis of Clycoconjugates Table 5.2 Enzymes used in modules for combinatorial biocatalysis of nucleotide sugars. 

In summary, the synthesis and in situ regeneration of nucleotide sugars by combinatorial biocatalysis suffers from the main disadvantage that each enzyme has to be produced in sufficient amounts. This affords efficient recombinant protein produchon hosts being a bottleneck for some genes [25]. However, once a multi-enzyme system has been developed, the productivity can be improved by repetitive use of the biocatalysts as demonstrated for repetitive batch syntheses with soluble enzymes [25, 38] or with immobilized enzymes [48]. The advantage... 

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