MS detection enhancement factor

MS detection enhancement factor = [conventional HPLC column diameter (4.6 mm)/(nano LC column diameter in mm)]2 (14.1)... 

For a 75 /.mi ID nano LC column as an example, the MS detection enhancement factor (ion count) in comparison to a 4.6 mm column is much higher than (4.6/0.075)2 = 3761 because of the reduction in sample molecular zone dilution and because a nano LC solvent flow rate at 0.02 to 2 /iL/min can be 100% directly sprayed into the MS ion source. No post-column flow splitting is required for nano-LC-MS as that required when 1 mL/min is used in a 4.6 mm ID column. This large enhancement of MS detection and the ability to directly interface with MS presents nano LC-MS as the best tool for life science research. 

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See also in sourсe #XX -- [ ]

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