Mouse hot-plate assay

Mininmin effective dose (MED) in mouse hot-plate assay, i.p. (294, 342). 

Endorphin Analogs - D-Ala, N-MeMet -enkephallnamide ( ) has been found to be a potent parenteral analgesic.59 it was 238 times as potent as normor-phlne in the mouse vas deferens assay, and four times as potent as morphine in the mouse hot plate assay (jump response) after subcutaneous administration. It is claimed that has relatively little respiratory depressant properties or tendency to cause tolerance or physical dependence. A series of Met -enkephalin analogs extended at the amino terminus with amino acid residues corresponding to 6-LPH have been described. Using the guinea-pig ileum-myenteric plexus assay, potency was found to decrease dramatically on further extension of the g-LPH 60-65 sequence... 

Table 7. Activity in Mouse Hot Plate Assay (Minimum Effective Dose, pmol/kg) for Pyridyl Ether Compounds of General...

B, Structure-Activity Studies - There is an Increasing tendency to use the newer tests (inflammed foot, writhing, bradykin) for evaluation of analgesic potency but, unless otherwise specified, potency estimates in the following discussion are based on the mouse hot plate assay. 

Wesolowska A, Young S, Dukat M. MD-354 potentiates the antinociceptive effect of clonidine in the mouse tail-flick but not hot-plate assay. Eur J Pharmacol 2004 495 129-136. 

Two of the more reliable laboratory methods for evaluating strong analgesics are the Bass-VanderBrook (I) modification of the D Amour-Smith (3) rat tail-flick method and the Eddy-Leimbach (6) variant of the mouse hot-plate technique. The tail-flick assay seems to be more specific for morphine-like drugs, since compounds which are positive in this test are also active in the... 

Probably the most commonly used in vivo bioassay is the mouse tetrad assay, in which the ability of a test compound to produce four effects in the same animal is determined. These effects, hypokinesia, hypothermia, catalepsy in the Pertwee ring test and antinociception in the tail-flick or hot plate test, are usually produced by a CBl receptor agonist over a relatively narrow dose range (reviewed in Howlett et al. 2002 Martin et al. 1995). One or other of these effects can be produced by some centrally active non-CBi receptor agonists or antagonists. However, when performed together, the tetrad tests provide at least some degree of... 

---