Mossbauer spectroscopy factors affecting

A further restriction is the detection limit. A reliable Mossbauer spectrum for studying a certain component in a mixture can be obtained only when its concentration is no less than 5-10% of the overall content of Mossbauer atoms of interest. Another limitation is the sensitivity. The correlation between the concentration of the Mossbauer atoms and the area under the spectral lines is ambiguous in some cases. When Mossbauer spectroscopy is used to study the analytical or conformational information of a certain sample, the above limitations should be taken into account and undesired factors which may affect results should be ruled out. More specifically, Mossbauer spectroscopy is more convenient in cases where either a complex system needs to be roughly studied in a short period, or the sample needs to be retained without destruction throughout analysis, or where other approaches are too costly for analysis. 

---