Monomer-template interactions factors affecting

It is of obvious importance that the functional monomers strongly interact with the template prior to polymerisation, since the solution structure of the resulting assemblies presumably defines the subsequently formed binding sites. By stabilising the monomer-template assemblies it is possible to increase the number of imprinted sites. At the same time the number of non-specific binding sites will be minimised, since there will be a reduction in the amount of free, non-associated functional monomer. For any particular template, the following factors that are likely to affect the recognition properties of the site have been identified (Fig. 5.14). 

---