Monolayers human amnion cells

Monolayer cultures of WISH (human amnion) cells were treated with the Indicated concentrations of each polymer. Toxic effects were recorded at the end of 24 hours. 4 = lOOt cell destruction, 3 = 751,... 

Using a genetic approach, Sharpe and Fields (1982) also have examined the effects of the mammalian reoviruses on host cell protein synthesis. Reovirus type 2 was found to inhibit protein synthesis in L cell monolayers more rapidly and efficiently than reovirus type 1 or 3. Type 2 reovirus also inhibits protein synthesis in human amnion cells (Loh and Soergel, 1967). The capacity of type 2 reovirus to... 

Purine metabolism in man is of increasing clinical and experimental interest, but studies of this subject have been hampered by methodological difficulties. This paper describes new methods for the measurement of apparent activities of more than 20 enzymes of purine metabolism in human leukocytes. These methods have also been applied to cultured human lymphoblasts and monolayer cultures of skin fibroblasts and amnionic cells. 

Mixed leukocytes are prepared using dextran sedimentation of erythrocytes, centrifugation of leukocytes from the plasma, and lysis of residual erythrocytes with ammonium chloride. Granulocytes and mononuclear leukocytes are separated from each other and from erythrocytes by density gradient centrifugation using a mixture of Ficoll, Hypaque and dextran. Thirty ml of blood provides sufficient leukocytes for study of adenine, guanine and hypoxanthine metabolism in duplicate. Suspension cultures of human lymphoblasts and leukemic cells, and monolayer cultures of skin fibroblasts and amnionic cells were studied under normal culture conditions approximately 10 cells are required for each incubation. 

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