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Monoclonal antibody screening principles

The basic principle for making the rabbit monoclonal antibody is the same as for mouse monoclonals. Rabbit fusion partner cells can fuse to rabbit B-cells to create the rabbit hybridoma cells. Hybridomas are then screened to select for clones with... [Pg.8]

Irving RA, Hudson PJ, Coding JW (1996), Construction, screening and expression of recombinant antibodies, In Goding JW (Ed.), Monoclonal Antibodies Principles and Practice, 3rd Ed., Academic Press, Manchester, pp. 424-464. [Pg.431]

In a protein-binding assay with fluorescence detection a microarray of biotin, HPYPP-peptide and WSHHPQFEK-peptide was screened against streptavidin-Cy3 and avidin-Cy5. By following the same principle an anti-human insulin monoclonal antibody was also screened against a set of different peptides. [Pg.495]

See other pages where Monoclonal antibody screening principles is mentioned: [Pg.2131]    [Pg.596]    [Pg.561]    [Pg.227]    [Pg.241]    [Pg.232]    [Pg.62]    [Pg.227]    [Pg.489]    [Pg.241]    [Pg.335]   
See also in sourсe #XX -- [ Pg.241 ]



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Monoclonal antibodies principle

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