Monoclonal antibodies mutant, production

The polypeptide patterns of greening barley indicate that these PSI - polypeptides are S5mthesized diiring the first six hours of gree-ning(15). Therefore, poly-A+ and plastid RNA were isolated and the presence of the transcripts for SUII, the SUIII and the Chl - apoprotein 1 was studied by tn vitvo translation and following immunoprecipitation with monoclonal antibodies. In the wild - type as well as in the mutant these transcripts are present (FIG.Ia-c). The translation products for the SUII and SUIII show an apparent of about 23 and 30 kD respectively and for the Chl -apoprotein 1 an apparent of about 69 kD. 

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