Monoamine measurements, electrochemical

ELECTROCHEMICAL DETECTION METHODS EOR MONOAMINE MEASUREMENTS IN VITRO AND IN VIVO... 

Measurements of octopamine in insects have almost exclusively been made using the radioenzymatic assay. However, recent advances have now resulted in the availability of high performance liquid chromatography coupled with electrochemical detection for the measurements of catecholamines, monoamines and their metabolites (20,21 ). This technique affords the advantage of estimating several compounds simultaneously in a single sample. One may anticipate an increase in the use of this technique in the near future. 

Serotonin In blood serotonin is predominantly located in the platelets, with levels in plasma being 100-fold lower. Most analysts interested in serotonin measurements have concentrated on determining serotonin in whole blood or platelet rich plasma. The presence of monoamine oxidase enzymes in blood necessitates the use of an enzyme inhibitor, e.g., pargyline to prevent the conversion of serotonin to 5-HIAA prior to analysis. Similar chromatographic conditions to those used for catecholamines can be used, e.g., CIS reversed-phase columns and electrochemical detection at an oxidation potential of -I-0.75 V or fluorescence detection. Sample... 

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