Monitoring Release of Neurotransmitter

Resuspend 1 mg of synaptosomes with 1 ml of prewarmed assay buffer and stir the solution with a magnetic stirrer in a water bath at 37 C for 15 min. 

Transfer synaptosomal suspension to a cuvette, place the cuvette in the thermoequilibrated chamber and stir. 

Unless added previously, add Ca or EGTA to final concentrations of 1.3 mM or 0.5-1 mM respectively. 

Add NADP and glutamate dehydrogenase, switch on the recorder and wait for a flat baseline (it can take 1 to 5 min). 

Trigger exocytosis by adding KCI to a final concentration of 30-50 mM. Record for 5-10 min. 

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