Molecular composition capillary zone electrophoresis

Capillary zone electrophoresis (CZE), micellar capillary electrokinetic chromatography (MECC), capillary gel electrophoresis (CGE), and affinity capillary electrophoresis (ACE) are CE modes using continuous electrolyte solution systems. In CZE, the velocity of migration is proportional to the electrophoretic mobilities of the analytes, which depends on their effective charge-to-hydrodynamic radius ratios. CZE appears to be the simplest and, probably, the most commonly employed mode of CE for the separation of amino acids, peptides, and proteins. Nevertheless, the molecular complexity of peptides and proteins and the multifunctional character of amino acids require particular attention in selecting the capillary tube and the composition of the electrolyte solution employed for the separations of these analytes by CZE. 

Carbohydrate analysis using enzymatic hydrolysis and subsequent capillary zone electrophoresis (ENZ/CZE) revealed the number of 4-0-methylglucuronic acid units to be approximately one unit per nineD-xylose residues (Table 4). No glucuronic acid units were detected. The carbohydrate composition of the polysaccharide isolated here from aspen wood is quite similar to that previously reported for typical hardwood xylans . SEC analysis indicated the molecular weight (Mw) to be 15000 g/mol. 

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