Molecular biology production

A survey on molecular biology products (Ausubel et at, 1991) indicated that for nitrocellulose about 70% of respondents preferred S S NC (other manufacturers < 10%) but that the preference for nylon membranes differed little among Schleicher Schuell, Dupont NEN and Amersham. In the same survey, respondents had no clear preference for either nylon or nitrocellulose for Southern hybridization but a clear preference for nitrocellulose in the case of colony/plaque hybridization (where concentration of target is sufficient highly but background should be suppressed). 

Rice-Evans, C.A., Diplock, A.T. and Symons, M.C.R. (1991). Mechanisms of radical production. In Laboratory Techniques in Biochemistry and Molecular Biology , Vol. 22, Techniques in Free Radical Research , (eds. R.H. Burdon and P.H. van Knippenberg) pp. 19-50. Elsevier, Amsterdam. 

Fluorophosphates (phosphorfluoridates), which are important for backbone modified oligonucleotides in molecular biology, are generally synthesized from phosphoric imi-dazolides or triazolides and acylfluorides. In these reactions, for example, the azole group (of the phosphoric or phosphinic imidazolide) is substituted by a fluorine atom, forming an acylazole compound as by-product.[202],[2033... 

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