Molar mass from Zimm plot

For MALS measurement foUowing GPC separation, the sample concentration at the LS detector is usually diluted sufficiently that the term 2A2C often may be safely dropped from Eq. 1. In some applications involving very high molar masses, it is often worthwhile to perform an off-line determination of the second virial coefficient from a Zimm plot to confirm its neghgible effect on the derived molar mass of Eq. 1. 

With MALS detectors, the molar mass for every elution volume slice is obtained from a partial Zimm, Debye, or Berry plot, where the scattering intensity of aU angles is... 

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