Mobile phase strength, organic modifier type and

The aqueous mobile phase conditions, e.g., buffer pH, type of buffer, ionic strength, nature and concentration of competing ligands, type and amount of organic modifier and temperature, also influence the overall observed enantioselectivity of CLEC systems. 

Retention can be influenced by the type and the concentration of the pairing reagent, the ionic strength, and the pH of the mobile phase, as well as the concentration and type of the organic modifier to the mobile phase. The technique therefore opens the window for additional parameters that can be used to influence the selectivity of a separation. 

Once a selection of the column type has been made, sample resolution is optimized by adjusting the ionic strength, pH, temperature, flow rate, and concentration of buffer or organic modifier in the mobile phase [39a], The influence of these parameters on solute retention is summarized in Table 15.9. 

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