Super-resolution microscopy

However, a few techniques exist that make it possible to circumvent the diffraaion limit and enhance the resolution. This will allow to image details that cannot be seen with conventional fluorescence microscopy.Super-resolution microscopy so far is mainly used in biology, for imaging subcdlular compartments and stmctures. But also for polymers, super-resolution microscopty can contribute to a better understanding of their properties. Just as an example, it is well known that the photophysical prop>erties of conjugated polymers depend strongly on thdr local conformation. With super-resolution microscopy, it will be possible to obtain detailed information about this conformation. [Pg.501]

Bates M, Huang B, Zhuang X (2008) Super-resolution microscopy by nanoscale localization of photo-switehable fluorescent probes. Curr Opin Chem Biol 12 505-514... [Pg.143]

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Shetengel et al, Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proc. Natl. Acad. Sci. USA 106, 3125-3130 (2009)... [Pg.415]

Super-Resolution Microscopy by Two-Color Double Resonance Spectroscopy... [Pg.289]

Figure29.2 Concept of super-resolution infrared be smaller than the diffraction limit of IR or microscopy based on TFD-IR spectroscopy. visible light, (b) Co-linear visible and IR light (a) Principle of super-resolution infrared beams are introduced to the sample,...

$Figure29.2 Concept of super-resolution infrared be smaller than the diffraction limit of IR or microscopy based on TFD-IR spectroscopy. visible light, (b) Co-linear visible and IR light (a) Principle of super-resolution infrared beams are introduced to the sample,...$

We have performed super-resolution infrared microscopy by combining a laser fluorescence microscope with picosecond time-resolved TFD-IR spectroscopy. In this chapter, we have demonstrated that the spatial resolution of the infrared microscope improved to more than twice the diffraction limit of IR light. It should he relatively straightforward to improve the spatial resolution to less than 1 pm by building a confocal optical system. Thus, in the near future, the spatial resolution of our infrared microscope will be improved to a sub-micron scale. [Pg.302]

Furthermore, this new super-resolution infrared microscopy is capable of capitalizing on TFD-IR spectroscopy s ability to study vibrational relaxation processes. [Pg.302]

Therefore, by using this super-resolution infrared microscope, we will be able to carry out space- and time-resolved vibrational microspectroscopy in the IR super-resolved region. Given that IR absorption is regarded as the fingerprint of a molecule, the new super-resolution infrared microspectroscopy will become an extremely important tool, not only in microscopy but also in spectroscopy. [Pg.303]

Sakai, M., Kawashima, Y., Takeda, A., Ohmori, T. and Fujii, M. (2007) Far-field infrared super-resolution microscopy using picosecond time-resolved transient fluorescence detected IR spectroscopy. Chem. Phys. Lett., 439, 171—176. [Pg.305]

Huang B, Bates M, Zhuang X (2009) Super resolution fluorescence microscopy. Ann Rev Biochem 17 993-1016... [Pg.1099]

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