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Microscopic fluorescence assay detection systems

Measuring FRET by fluorescence lifetime imaging microscopy (FRET-FLIM) offers the ability to see beyond the resolution of the optical system ( 10-100 times that of modern far field microscopes [5]). FRET efficiency can be used as a proxy for molecular distance, thereby allowing the easy detection and somewhat more challenging quantification of molecular interactions. Although many types of assay exist, FRET-FLIM is a highly suitable technique that is capable of in situ measurements of molecular interactions and conformation in living and fixed cells. [Pg.459]

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Assay systems

Detection systems

Fluorescence assay

Fluorescence detection

Fluorescence microscopes

Fluorescence-detected

Microscopic fluorescence assay

Microscopic systems

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