Microcalorimetry, experimental procedures

Experimental procedures for running excipient compatibility studies using isothermal microcalorimetry include the collection of power-time curves for each component of the mixture alone, as well as in combinations (Fig. 8). The separate drug and excipient curves can then be used to construct a theoretical non-interaction curve for the blend, which then is subtracted from the actual blend curves in order to define the interaction between the components. 

Because of the appreciable enhancement of the adsorption potential in micropores of a few molecular diameters in width (see Section 1.7 and Figure 1.6), microcalorimetry can provide a useful means of assessing microporosity. The available experimental procedures are outlined in the following sections. 

Despite the great diversity in the design of microcalorimeters and the experimental procedures described in the literature [1-10], only two microcalorimetric methods have found widespread application in cydodextrin (CyD) studies and drug-design research. These two methods are differential scanning calorimetry (DSC) and isothermal titration microcalorimetry (ITC). DSC and ITC can be con-... 

Fig. 6. Investigation of kinetic properties of immobilized invertase by flow microcalorimetry in the circulation mode. Initial sucrose concentration 51 mM, invertase immobilization by biospecific binding on concanavalin A-bead cellulose was prepared by binding on concana-valin A linked to chlorotriazine-activated cellulose, a Raw experimental thermometric data b data after conversion by the procedure indicated in Fig. 4. Concentrations were determined spectrophotometrically (open symbols) and by transformation of thermometric data explained in Section 5 (closed symbols) [32]...

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