Michaelis-Menten worked examples

The problem is that substrate conversions are frequently lower than 10% and thus difficult to detect in most traditional formats. As previously discussed in this chapter, /iPLC allows optimal operation even when working with low substrate conversion (e.g., as low as 1% as described by Wu et al.12). Data obtained allow the calculation of the Michaelis-Menten constant (Km) for ATP. An example of such an evaluation is presented in Figure 6.48 in which the obtained reaction velocities... 

These problems were overcome by immobilizing the enzyme. Since the usual methods for immobilization of laccase did not work, we adopted a new method, details of which will be described elsewhere (29). Reasonable measurements were possible with this technique. Typical patterns of laccase activity could be monitored via the changes of absorbance of 2,6 DMP and syringaldazine. When the reaction took place in organic solvents, the absorption spectra of the products were similar to those obtained for the same reaction in buffer. Furthermore, the catalytic action of the T. versicolor laccase followed Michaelis-Menten-kinetics in most of the organic solvents which were tested (see Table II for specific examples). 

In this section we discuss some examples of relatively complex catal)dic systems based on electroactive polymers. Initially we discuss a complex substrate reaction that can be described in terms of Michaelis-Menten kinetics. We then describe recent work conducted on catalytic systems using immobilized enzymes in electronically conducting polymer films. We end by describing microheterogeneous systems containing... 

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