Michaelis-Menten equation graphic representations

Figure 2.12 Graphical representation of the Michaelis-Menten equation for nonallosteric enzymes.

GRAPHICAL REPRESENTATION. The above expression represents the equation of a hyperbola (i.e., f(x) = axl(b + x) where a and b are constants) and a plot of the initial velocity as a function of [S] will result in a rectangular hyperbola. Another way for representing the Michaelis-Menten equation is by using the doublereciprocal (or, Lineweaver-Burk ) transformation ... 

Graphical transformation of the representation of enzyme kinetics is useful as the value of V max is impossible to obtain directly from practical measurements. A series of graphical transformations/linearisations may be used to overcome this problem. Lineweaver and Burk (see reference(,7)) simply inverted the Michaelis-Menten equation (equation 5.10). Thus ... 

FIGURE 1.2 Graphic representations of Michaelis-Menten equation, (a) Michaelis—Menten equation, (b) Lineweaver-Burk plot, and (c) Eadie—Hofstee plot. 

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