360-MHz proton NMR

Figure 1 360 MHz Proton NMR spectrum, in CDC13, of protoporphyrin-IX (19) dimethyl ester. Assignments a, methine protons b, vinyl CH c, vinyl CH2 d, propionate CH2CH2CO e, nuclear methyls and methyl esters f, propionate CH2CH2CO g, NH...

Figure 5. 360-MHz proton NMR spectrum of Forrsman hapten in DMSO-ds...

Melting Transition Typical 360 MHz proton NMR spectra of proflavine poly(dA-dT) complexes, Nuc/D = 24 and Nuc/D = 8, in 1 M NaCl solution at temperatures below the midpoint for the dissociation of the complex are presented in Figures 17A and B respectively. The stronger base and sugar resonances can be readily resolved from the weaker proflavine resonances (designated by asterisks) in the presence of excess nucleic acid (Figure 17) so that the resonances of the synthetic DNA and the mutagen can be monitored independently of each other. 

Figure 17. The 360-MHz proton NMR spectra of the proflavine poly(dA-dT) complex in 1M NaCl, lOmM cacodvlate, JOmM EDTA, zHzO, pH 7. The top spectrum represents the Nuc/D = 24 complex at 78.5°C ( ut of the proflavine resonances in the complex is 80°C), while the bottom spectrum represents the Nuc/D = 8 complex at 8l.4°C ft,/, of proflavine resonances in complex is 84.3°C). The proflavine resonances are designated by asterisks.

Figure 26. The temperature dependence of the 360-MHz proton NMR spectra (4.5 to 9.0 ppm) of the daunomycin poIy(dA-dT) complex, Nuc/D =25, in /M NaCl, lOmM cacodylate, ImM EDTA, 2H.O, pH 6.5 between 69° and 88°C. The daunomycin resonances are designated by an asterisk.

Figure 35. The temperature dependence (first heating) of the 360-MHz proton NMR spectra (5 to 9 ppm) oj the netrop-sin poly(dA-dT) complex, Nuc/D — 50, in 0.1 cacodylate, 4.4m5A EDTA, 2HtO, pH 7.5 between 53° and 68.5°C. The asterisks designate some of the minor resonances from base pairs centered at the binding site.

Figure 41. The 360-MHz proton NMR spectrum (4.5 to 9.0 ppm) of the Nuc/D = 10 netropsin poly(dA-dT) complex in 0.1 M cacodylate, 4.4mM EDTA 2H 0, pH 7.08 at 87°C. The tentatively assigned N-methylpyrrole protons of netropsin are...

Figure 8. A, A 360-MHz proton NMR spectrum of camphor, 2 M in deutero-chloroform B and C, A 55-MHz natural-abundance NMR spectrum of the same sample with (B), and without (C) proton broadband decoupling.

Figure 1. The 360 MHz proton NMR spectr of gene-5 protein in D2O recorded at 3l C. The peak indicated with an asterisk is from EDTA.

Figure 7. The effect of ApA binding upon the photp-CIDNP difference spectrum of the aromatic part of the 360 MHz proton NMR spectrum of gene-5 protein. Ratio ApA/gene-5 pro-telni (A) 0.0 (B) 4.6 (C) 11.4 (0) 17.3.

G. Wider, R. Baumann, K. Nagayama, R.R. Ernst, and K. Wuthrich, Strong spin-spin coupling in the two-dimensional J-resolved 360-MHz proton NMR spectra of the common amino acids, J. Magn. Resonance 42, 73 (1981). 

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