Methyl phenyl sulfide, alternative

An alternative method for coupling enzymes is to employ a mixed enzyme bioreactor Pezzotti and Therisod [60] reported a bienzymatic system in which alcohol oxidase and peroxidase were coupled to effect the enantioselective oxidation of the sulfide thioanisole (Scheme 3.4). Here, the peroxidase from Coprinus dnereus was mixed with a crude extract of Pichia pastoriz alcohol oxidase and the two enzyme mixture was successfully used to convert gram quantities of thioanisole enantiose-lectively to S-methyl-phenyl-sulfoxide with an enantiomeric excess of 75%. 

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