Methoxy-2,4-diphenyl-3 2H -furanone Reagent

Dipping solution Dissolve 25 mg 2-methoxy-2,4-diphenyl-3(2H)-furanone in 50 ml methanol. 

Storage The dipping solution may be stored for several days. 

MDPF reacts directly with primary amines to form fluorescent products. Secondary amines yield nonfluorescent derivatives, which may be converted into fluorescent substances by a further reaction with primary amines [2]. 

The chromatograms are freed from mobile phase in a stream of warm air, immersed in the reagent solution for 4 s or sprayed evenly with it and then heated to 110°C for 20 min. 

Yellow chromatogram zones are formed on a colorless background these exhibit yellow fluorescence in long-wavelength UV light (2 = 365 nm). 

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