Method 2 in Scheme

Ohga et al. cross-linked chitosan with epichlorohydrin to give an imprinted resin [7]. The obtained resin was utilised in column chromatography for separating metal ions. The column packed with a Cd(II)-imprinted resin could separate Cd(II), 

Imprinting polymerisation for recognition and separation of metal ions 

Cu(II) and Hg(II). This separation was achieved with 95-100% recovery by using a small column (8.0 x 0.4 cm i.d.). In addition, almost identical chromatograms were obtained when the metal ions were loaded into the column again after complete washing with deionised water, indicating an effective durability in repeated use. 

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EARLY STUDIES ON METAL ION-IMPRINTING 9.3.1. Method 1 in Scheme 9.1... 

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