Methanogens inhibitors

Methanogens catalyze this reaction if methanogenesis was blocked by inhibitors of the CH3-S-C0M reductase [105,106],... 

Heterodisulfide (CoM-S-S-HTP) reduction - coupled to primary translocation. The reaction in which ATP is synthesized during methanol reduction to CH4 could be identified with energetically competent membrane vesicles of the methanogenic strain G61. These vesicles, which are orientated more than 90% inside-out, catalyzed CH4 formation from CH3-S-C0M by reduction with H2 (Reactions 7,8) and coupled this process with the synthesis of ATP [112]. CH3-S-C0M reduction generated a ApH (inside acidic) as monitored by acridine dye quenching protonophores and ATP synthase inhibitors exerted their effects in accordance with a chemiosmotic type of ATP synthesis [113],... 

Components of the archaeal translation apparatus, however, display an intra-domain diversity, not encountered within the other two domains. This situation is exemplified by (i) the lack of a uniform compatibility between ribosomes and elongation factors from different archaeal lineages (see section 5.2) (ii) the impressive diversity of archaeal ribosomes and factors in their response to a host of protein synthesis inhibitors (see section 4) (iii) the heterogeneity in shape, mass and composition of archaeal ribosomal subunits (see sections 2.6.2, 2.6.3). Importantly, within-domain diversity is also exemplified by the different complexities of the RNA polymerase subunit patterns from the sulfur-dependent and the methanogenic-halophilic archaea (see Zillig et ah. Chapter 12 of this volume). 

Also, the methane fermentation of formate by M. omelianskii is almost completely abolished by hypophosphite (22), a selective inhibitor of the formic hydrogenylase of the non-methanogenic symbiont (23). However, in the residual fermentation by the methanogenic component, some transfer of deuterium from the substrate to methane indicates a very limited amount of direct reduction ... 

They were used to delineate an unusual pathway for the degradation of 3-methylphenol carried out by a methanogenic consortium (Londry and Fedorak 1993). Three compounds were used as selective inhibitors ... 

De Graaf, W., Wellsbury, P., Parkes, R.J., and Cappen-berg, T.E., 1996. Comparison of acetate turnover in methanogenic and sulfate-reducing sediments by radio- and stable-isotope labeling and specific inhibitors evidence for isotopic exchange. Appl. Env. Microbiol., 62 772-777. 

Another environmental parameter that is important to methane emissions is soil temperatnre. Soil temperature affects the metabolic activity of microorganisms that prodnce substrates for inhibitors of methanogenesis as well as the methanogens themselves. Temperatnre is directly related to methanogenesis under field conditions (Schutz et al., 1990 Westermann, 1993). Methane emission rates generally parallel soil temperature levels. 

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